Nws yog cov nquag siv eukaryotic receptor cell hauv noob qes cloning thwmsim, thiab nws yooj yim rau kev sau cov poov xab li kuaj kab mob E. coli. Kuj tseem muaj ntau hom poov xab cloning vectors. Poov tseem muaj plasmids. Qhov 2μm ntev plasmid no yog hu ua 2μm plasmid, txog 6 300bp. Qhov no plasmid muaj nyob sab nraum lub chromosomes hauv lub keeb hauv tsawg kawg ib lub sijhawm. Qhov 2μm plasmid thiab E. coli plasmid tuaj yeem siv los tsim lub shuttle plasmid uas tuaj yeem shuttle ntawm cov kab mob thiab cov poov xab hlwb. Poov xab cloning vectors tau tsim txhua qhov no.
Poov xab yog cov noob qe muaj ib hom, tsis yog chav nyob ntawm kev faib tawm phylogenetic. Ib qho me me hauv cov kab mob me me uas pom tsis tau cov ntsej muag liab qab. Nws tuaj yeem tso suab thaj rau hauv dej cawv thiab carbon dioxide. Nws tau faib rau txhua qhov. Nws yog hom kab mob kev txawj ntse heterotrophic facultative anaerobic microorganism tuaj yeem ua neej nyob ob leeg aerobic thiab anaerobic. Nws yog ib hom tshuaj tsw qab ua rau neeg sawv cev.