Acid dyes muaj auxochromatic pawg xws li hydroxyl, carboxyl, thiab sulfone pawg uas tsis zoo rau hauv kev daws teeb meem. Cov ntsev uas muaj cov xim no thiab Na, K, thiab Ca ions yog alkaline hauv kev daws. Nws ua ke nrog cov khoom tseem ceeb ntawm cov hlwb zoo, los yog nkag mus rau hauv qhov chaw intercellular kom stain cytoplasm, qe ntshav liab, nqaij fibers, thiab lwm yam.
