Kev Ua Tau Zoo Kua Chromatography (HPLC)

Txawm hais tias ob lub ntsej muag ntawm gel electrophoresis (2-DE) yog cov qauv siv rau kev txheeb xyuas tag nrho cov tshuaj tiv thaiv, nws muaj cov kev txwv xws li kev muaj peev xwm sib cais, kev ua haujlwm tsis zoo, thiab cov txheej txheem ua haujlwm nyuaj. Nws yog feem ntau nyuaj kom tau txais kev txaus siab rau kev soj ntsuam ntawm ntau qhov kev hloov pauv loj, tsis muaj ntau thiab cov protein hydrophobic. Chong li al. siv HPLC / mass spectrometry los sib piv thiab tshawb xyuas qhov sib txawv ntawm cov lus qhia ua ntej-malignant thiab qog nqaij hlav cancer. Cov protein tau sib cais los ntawm HPLC, thiab cov zauv sau sib cais tau txheeb xyuas los ntawm MALDI-TOF-MS, kom cov protein muaj cov roj ntau txheeb rau hauv qhov sib txawv ntawm ob hom hlwb. Raws li hom tshiab ntawm kev sib cais siv tshuab, multidimensional kua nyeem ua kua tsis muaj kev txwv ntawm qhov txheeb ze molecular thiab isoelectric point. Los ntawm kev sib koom ua ke ntawm cov qauv sib txawv, nws tshem tawm cov kev sib cais ntawm ob-txheej gel electrophoresis. Nws muaj cov yam ntxwv ntawm siab siab muaj peev xwm thiab automation yooj yim. Ob-dimension ion pauv-thim rov qab theem chromatography (2D-IEC-RPLC) yog cov feem ntau siv los ua kua-kua kua kua nyeem ua kua hluav taws xob sib cais muaj nyob rau hauv kev tshawb nrhiav proteomics.