Lub sij hawm-ntawm-lub davhlau loj spectrometry

Lub ntsej muag-ua kom zoo laser desorption ionization sij hawm ntawm kev ya davhlau loj spectrometry thev naus laus zis tau tsim los ntawm Nobel nqi zog Tanaka nyob rau xyoo 2002, thiab nws tau nyiam zoo los ntawm kev kawm hauv zej zog tom qab nws tau tsim tawm. SELDI technology yog lub thev naus laus zis zoo tshaj plaws hauv kev tshawb nrhiav proteomics. Nws lub npe tag nrho yog saum npoo-kho kom zoo laser desorption / ionization sijhawm-ntawm-davhlau loj spectrometry (SELDI-tof). Cov txheej txheem tseem ceeb yog raws li hauv qab no: nyob rau hauv ib txwm muaj tshwm sim, tus qauv yog ncaj qha lawb mus rau qhov tshwj xeeb hloov nti tom qab kev twv ua ntej, uas tuaj yeem piv cov protein sib txawv nruab nrab ntawm ob qho qauv thiab tau txais cov ntsiab lus ntawm protein ntau ntawm cov qauv. Yog li ntawd, nws muaj qhov zoo tseem ceeb hauv kev siv. Cov qauv ntsuas los ntawm SELDI thev naus laus zis tsis tas yuav raug kuaj ua ntej los ntawm cov kua nyeem ua kua lossis roj chromatography, yog li nws tuaj yeem siv los soj ntsuam cov roj ntsha mus kuaj. SELDI thev naus laus zis tuaj yeem soj ntsuam hydrophobic cov protein, cov protein sib luag nrog PI ntau dhau lossis tsis dhau, thiab cov roj ntsha muaj cov roj nyeem qis (GG lt; 25 000). Nws tseem tuaj yeem pom ntau cov roj ntsha muaj protein tsawg uas yog ua kom npog ntsej muag hauv kev ntsuas tsis tau, ua rau kev tshawb pom ntawm biomarkers Cov Sijhawm. SELDI thev naus laus zis tsuas yog kuaj ntshav me me xwb, cov txiaj ntsig tuaj yeem tau txais nyob rau lub sijhawm luv luv, thiab kev ntsuas tau rov ua dua tshiab, tsim nyog rau kev kuaj mob thiab kuaj ntsuas kab mob ntau yam, tshwj xeeb nws tuaj yeem tshawb pom cov ntshav tsis tau tso ntshav, ntshav, cerebrospinal kua, sib koom ua ke synovial kua, bronchial effluent, lysate ntawm tes thiab ntau yam kev zais, thiab lwm yam, kom lub molecular phaus, PI, glycosylation site, phosphorylation site thiab lwm yam tsis muaj txiaj ntsig ntawm lub hom phiaj protein hauv qhov qauv tuaj yeem kuaj tau.